Gene:  TNNI1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI) along with troponin T (TnT) and troponin C (TnC) is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch TnI-skeletal-slow-twitch and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers respectively while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: TNNI1, Human
troponin I type 1 (skeletal, slow)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013035
Info:   Same primer pair as used in probe assay qHsaCIP0028700
List Price:    $125.00
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PrimePCR™ Probe Assay: TNNI1, Human
troponin I type 1 (skeletal, slow)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028700
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: TNNI1, Human
troponin I type 1 (skeletal, slow)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019055
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013035; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TNNI1, Human
troponin I type 1 (skeletal, slow)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051136
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028700; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TNNI1, Human
troponin I type 1 (skeletal, slow)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051137
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028700; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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