Gene:  TMEM185A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is predicted to be a transmembrane protein but this has not been experimentally determined. This gene is better known for localizing to the CpG island of the fragile site FRAXF. The 5-prime untranslated region of this gene contains a CGG trinucleotide repeat sequence that normally consists of 7-40 tandem CGG repeats but which can expand to greater than 300 repeats. Methylation of the CpG island leads to transcriptional silencing of this gene but neither the silencing nor an expanded repeat region appear to manifest itself in a clear phenotypic manner. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2010]

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PrimePCR™ SYBR® Green Assay: TMEM185A, Human
transmembrane protein 185A

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012362
Info:   Same primer pair as used in probe assay qHsaCIP0028414
List Price:    $125.00
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PrimePCR™ Probe Assay: TMEM185A, Human
transmembrane protein 185A

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028414
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: TMEM185A, Human
transmembrane protein 185A

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018582
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012362; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TMEM185A, Human
transmembrane protein 185A

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050660
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028414; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: TMEM185A, Human
transmembrane protein 185A

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050661
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028414; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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