Gene:  SYNE1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a spectrin repeat containing protein expressed in skeletal and smooth muscle and peripheral blood lymphocytes that localizes to the nuclear membrane. Mutations in this gene have been associated with autosomal recessive spinocerebellar ataxia 8 also referred to as autosomal recessive cerebellar ataxia type 1 or recessive ataxia of Beauce. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SYNE1, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044355
Info:   Same primer pair as used in probe assay qHsaCEP0051617
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PrimePCR™ SYBR® Green Assay: SYNE1, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010950
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: SYNE1, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051617
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PrimePCR™ ddPCR™ Mutation Assay:SYNE1 p.R8468H, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502326
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SYNE1 WT for p.R8468H, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502327
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SYNE1 p.R4223H, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503370
Info:   96nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SYNE1 WT for p.R4223H, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503371
Info:   96nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SYNE1 p.L5507P, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504116
Info:   66nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SYNE1 WT for p.L5507P, Human
spectrin repeat containing, nuclear envelope 1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504117
Info:   66nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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