Gene:  STK11, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene which encodes a member of the serine/threonine kinase family regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract pigmented macules on the skin and mouth and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: STK11, Human
serine/threonine kinase 11

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0008647
Info:   Same primer pair as used in probe assay qHsaCIP0026892
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PrimePCR™ Probe Assay: STK11, Human
serine/threonine kinase 11

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026892
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PrimePCR™ ddPCR™ Copy Number Assay: STK11, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000502
Info:   69nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:STK11 p.Q170*, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501400
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:STK11 WT for p.Q170*, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501401
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:STK11 p.D194Y, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502310
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:STK11 WT for p.D194Y, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502311
Info:   64nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:STK11 p.Y272Y, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503348
Info:   61nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:STK11 WT for p.Y272Y, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503349
Info:   61nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:STK11 p.F354L, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504282
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:STK11 WT for p.F354L, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504283
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:STK11 p.Q37*, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505558
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:STK11 WT for p.Q37*, Human
serine/threonine kinase 11

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505559
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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