Gene:  SPTLC3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 2.3.1.50) which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al. 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3) depending on the tissue in which biosynthesis occurs (Hornemann et al. 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit SSSPTA (MIM 613540) and SSSPTB (MIM 610412) that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al. 2009 [PubMed 19416851]).[supplied by OMIM Nov 2010]

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PrimePCR™ SYBR® Green Assay: SPTLC3, Human
serine palmitoyltransferase, long chain base subunit 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046891
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0054152
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: SPTLC3, Human
serine palmitoyltransferase, long chain base subunit 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012276
List Price:    $125.00
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PrimePCR™ Probe Assay: SPTLC3, Human
serine palmitoyltransferase, long chain base subunit 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0054152
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPTLC3, Human
serine palmitoyltransferase, long chain base subunit 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011276
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046891; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPTLC3, Human
serine palmitoyltransferase, long chain base subunit 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018524
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012276; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPTLC3, Human
serine palmitoyltransferase, long chain base subunit 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040862
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0054152; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPTLC3, Human
serine palmitoyltransferase, long chain base subunit 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040863
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0054152; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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