Gene:  SPOP, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX) which interacts with histone deacetylase core histones and other histone-associated proteins. In mouse the encoded protein binds to the putative leucine zipper domain of macroH2A1.2 a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SPOP, Human
speckle-type POZ protein

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0033986
Info:   Recommended - best efficiency
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PrimePCR™ SYBR® Green Assay: SPOP, Human
speckle-type POZ protein

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043893
Info:   Same primer pair as used in probe assay qHsaCEP0051155
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PrimePCR™ Probe Assay: SPOP, Human
speckle-type POZ protein

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051155
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PrimePCR™ ddPCR™ Mutation Assay:SPOP p.F133V, Human
speckle-type POZ protein

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501534
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SPOP WT for p.F133V, Human
speckle-type POZ protein

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501535
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SPOP p.F133L, Human
speckle-type POZ protein

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505534
Info:   72nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SPOP WT for p.F133L, Human
speckle-type POZ protein

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505535
Info:   72nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SPOP p.F133L, Human
speckle-type POZ protein

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505616
Info:   72nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SPOP WT for p.F133L, Human
speckle-type POZ protein

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505617
Info:   72nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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