Gene:  SPAM1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Hyaluronidase degrades hyaluronic acid a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte a receptor that plays a role in hyaluronic acid induced cell signaling and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2010]

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PrimePCR™ SYBR® Green Assay: SPAM1, Human
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017341
Info:   Same primer pair as used in probe assay qHsaCIP0031176
List Price:    $130.00
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PrimePCR™ Probe Assay: SPAM1, Human
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031176
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPAM1, Human
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022440
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017341; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPAM1, Human
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5055574
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031176; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPAM1, Human
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5055575
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031176; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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