Gene:  SPAG8, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility as well as identification of proteins for targeted contraception are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SPAG8, Human
sperm associated antigen 8

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0004227
Info:   Same primer pair as used in probe assay qHsaCEP0024968
List Price:    $125.00
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PrimePCR™ Probe Assay: SPAG8, Human
sperm associated antigen 8

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0024968
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPAG8, Human
sperm associated antigen 8

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5002513
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0004227; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPAG8, Human
sperm associated antigen 8

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5026892
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0024968; Exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPAG8, Human
sperm associated antigen 8

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5026893
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0024968; Exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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