Gene:  SPAG1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility as well as identification of proteins for targeted contraception are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: SPAG1, Human
sperm associated antigen 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043012
Info:   Same primer pair as used in probe assay qHsaCEP0050274
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: SPAG1, Human
sperm associated antigen 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007503
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: SPAG1, Human
sperm associated antigen 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050274
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPAG1, Human
sperm associated antigen 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007840
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043012; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SPAG1, Human
sperm associated antigen 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5015137
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007503; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPAG1, Human
sperm associated antigen 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033992
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050274; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SPAG1, Human
sperm associated antigen 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033993
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050274; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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