Gene:  SMARCB1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is part of a complex that relieves repressive chromatin structures allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SMARCB1, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042726
Info:   Same primer pair as used in probe assay qHsaCEP0049988
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PrimePCR™ SYBR® Green Assay: SMARCB1, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0021943
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PrimePCR™ Probe Assay: SMARCB1, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049988
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PrimePCR™ ddPCR™ Copy Number Assay: SMARCB1, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000520
Info:   58nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 p.R201*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500908
Info:   72nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 WT for p.R201*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2500909
Info:   72nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 p.R40*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502024
Info:   68nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 WT for p.R40*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502025
Info:   68nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 p.R158*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502236
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 WT for p.R158*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502237
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 p.R53*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503228
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 WT for p.R53*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503229
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 p.R377H, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504802
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 WT for p.R377H, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504803
Info:   70nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 p.Y47*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505112
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 WT for p.Y47*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505113
Info:   63nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 p.E216*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505634
Info:   79nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SMARCB1 WT for p.E216*, Human
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505635
Info:   79nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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