Gene:  SHANK3, Human

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This gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors ion channels and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Shank proteins also play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD) which is characterized by impairments in social interaction and communication and restricted behavioral patterns and interests. Mutations in this gene also cause schizophrenia type 15 and are a major causative factor in the neurological symptoms of 22q13.3 deletion syndrome which is also known as Phelan-McDermid syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified. [provided by RefSeq Mar 2012]

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PrimePCR™ SYBR® Green Assay: SHANK3, Human
SH3 and multiple ankyrin repeat domains 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0037026
Info:   Same primer pair as used in probe assay qHsaCIP0039750
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PrimePCR™ Probe Assay: SHANK3, Human
SH3 and multiple ankyrin repeat domains 3

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0039750
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SHANK3, Human
SH3 and multiple ankyrin repeat domains 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5025063
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0037026; Intron-spanning
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PrimePCR™ ddPCR™ Copy Number Assay: SHANK3, Human
SH3 and multiple ankyrin repeat domains 3

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500305
Info:   FAM; 61nt amplicon; exonic; FAM version of SHANK3 (dHsaCP2506483)
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Copy Number Assay: SHANK3, Human
SH3 and multiple ankyrin repeat domains 3

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506483
Info:   HEX; 61nt amplicon; exonic; HEX version of SHANK3 (dHsaCP2500305)
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Expression Probe Assay: SHANK3, Human
SH3 and multiple ankyrin repeat domains 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5058812
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0039750; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: SHANK3, Human
SH3 and multiple ankyrin repeat domains 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5058813
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0039750; Intron-spanning
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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