Gene:  SF3B1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b together with splicing factor 3a and a 12S RNA unit forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical tandem HEAT repeats that form rod-like helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: SF3B1, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043621
Info:   Same primer pair as used in probe assay qHsaCEP0050883
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PrimePCR™ SYBR® Green Assay: SF3B1, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014777
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PrimePCR™ Probe Assay: SF3B1, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050883
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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