Gene:  SF3B1, Human

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This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b together with splicing factor 3a and a 12S RNA unit forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical tandem HEAT repeats that form rod-like helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SF3B1, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043621
Info:   Same primer pair as used in probe assay qHsaCEP0050883
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PrimePCR™ SYBR® Green Assay: SF3B1, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0014777
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PrimePCR™ Probe Assay: SF3B1, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050883
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.K666N, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500812
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.K666N, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2500813
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.Y623C, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501044
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.Y623C, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501045
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.H662D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501778
Info:   92nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.H662D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501779
Info:   92nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.K666N, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501854
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.K666N, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501855
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.H662Q, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501978
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.H662Q, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2501979
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.R957Q, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502110
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.R957Q, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502111
Info:   65nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.R625H, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502176
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.R625H, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502177
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.E622D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502306
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.E622D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502307
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.K666M, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502408
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.K666M, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502409
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.E622D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502636
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.E622D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2502637
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.N626Y, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503076
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.N626Y, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2503077
Info:   80nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.K666R, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504804
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.K666R, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2504805
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.R625L, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505478
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.R625L, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505479
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.R625C, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505504
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.R625C, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505505
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.K666E, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505516
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.K666E, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505517
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.G742D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505936
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.G742D, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505937
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 p.K666T, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2506182
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SF3B1 WT for p.K666T, Human
splicing factor 3b, subunit 1, 155kDa

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2506183
Info:   69nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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