Gene:  SERF1A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The duplication region includes both a telomeric and a centromeric copy of this gene. Deletions of this gene the telomeric copy often accompany deletions of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients and so it is thought that this gene may be a modifier of the SMA phenotype. The function of this protein is not known; however it bears low-level homology with the RNA-binding domain of matrin-cyclophilin a protein which colocalizes with small nuclear ribonucleoproteins (snRNPs) and the SMN1 gene product. Alternatively spliced transcripts have been documented but it is unclear whether alternative splicing occurs for both the centromeric and telomeric copies of the gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SERF1A, Human
small EDRK-rich factor 1A (telomeric)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056816
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057921
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: SERF1A, Human
small EDRK-rich factor 1A (telomeric)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0020649
List Price:    $125.00
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PrimePCR™ Probe Assay: SERF1A, Human
small EDRK-rich factor 1A (telomeric)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057921
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SERF1A, Human
small EDRK-rich factor 1A (telomeric)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5023578
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0020649; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SERF1A, Human
small EDRK-rich factor 1A (telomeric)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189449
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0056816; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SERF1A, Human
small EDRK-rich factor 1A (telomeric)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192589
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0057921; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SERF1A, Human
small EDRK-rich factor 1A (telomeric)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192590
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0057921; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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