Gene:  SEPP1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SEPP1, Human
selenoprotein P, plasma, 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048843
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0056103
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PrimePCR™ SYBR® Green Assay: SEPP1, Human
selenoprotein P, plasma, 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0021902
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PrimePCR™ Probe Assay: SEPP1, Human
selenoprotein P, plasma, 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0056103
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PrimePCR™ ddPCR™ Mutation Assay:SEPP1 p.K181R, Human
selenoprotein P, plasma, 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501728
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SEPP1 WT for p.K181R, Human
selenoprotein P, plasma, 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501729
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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