Gene:  SEC63, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: SEC63, Human
SEC63 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010439
Info:   Same primer pair as used in probe assay qHsaCIP0027584
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PrimePCR™ Probe Assay: SEC63, Human
SEC63 homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027584
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PrimePCR™ ddPCR™ Mutation Assay:SEC63 p.A251A, Human
SEC63 homolog (S. cerevisiae)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501148
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:SEC63 WT for p.A251A, Human
SEC63 homolog (S. cerevisiae)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501149
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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