Gene:  SCN7A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes one of the many voltage-gated sodium channel proteins. For proper functioning of neurons and muscles during action potentials voltage-gated sodium channels direct sodium ion diffusion for membrane depolarization. This sodium channel protein has some atypical characteristics; the similarity between the human and mouse proteins is lower compared to other orthologous sodium channel pairs. Also the S4 segments which sense voltage changes have fewer positive charged residues that in other sodium channels; domain 4 has fewer arginine and lysine residues compared to other sodium channel proteins. Several alternatively spliced transcript variants exist but the full-length natures of all of them remain unknown. [provided by RefSeq Dec 2011]

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PrimePCR™ SYBR® Green Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044702
Info:   Same primer pair as used in probe assay qHsaCEP0051964
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009598
Info:   Recommended - best efficiency
List Price:    $125.00
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PrimePCR™ Probe Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051964
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009325
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044702; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016503
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009598; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Copy Number Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500302
Info:   FAM; 65nt amplicon; exonic; FAM version of SCN7A (dHsaCP2506480)
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506480
Info:   HEX; 65nt amplicon; exonic; HEX version of SCN7A (dHsaCP2500302)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036962
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051964; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: SCN7A, Human
sodium channel, voltage-gated, type VII, alpha

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036963
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051964; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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