Gene:  RPE65, Human

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This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein a soluble form called sRPE65 and a palmitoylated membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT) the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RPE65, Human
retinal pigment epithelium-specific protein 65kDa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043699
Info:   Same primer pair as used in probe assay qHsaCEP0050961
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: RPE65, Human
retinal pigment epithelium-specific protein 65kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017033
List Price:    $130.00
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PrimePCR™ Probe Assay: RPE65, Human
retinal pigment epithelium-specific protein 65kDa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050961
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RPE65, Human
retinal pigment epithelium-specific protein 65kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008454
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043699; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RPE65, Human
retinal pigment epithelium-specific protein 65kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022185
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017033; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RPE65, Human
retinal pigment epithelium-specific protein 65kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035220
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050961; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RPE65, Human
retinal pigment epithelium-specific protein 65kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035221
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050961; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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