Gene:  RFX5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B C and D all lead to a deficiency in RFX a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al. 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RFX5, Human
regulatory factor X, 5 (influences HLA class II expression)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045126
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052387
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: RFX5, Human
regulatory factor X, 5 (influences HLA class II expression)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0020224
List Price:    $125.00
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PrimePCR™ Probe Assay: RFX5, Human
regulatory factor X, 5 (influences HLA class II expression)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052387
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RFX5, Human
regulatory factor X, 5 (influences HLA class II expression)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009697
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0045126; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RFX5, Human
regulatory factor X, 5 (influences HLA class II expression)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5023509
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0020224; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RFX5, Human
regulatory factor X, 5 (influences HLA class II expression)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5037704
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0052387; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RFX5, Human
regulatory factor X, 5 (influences HLA class II expression)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5037705
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052387; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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