Gene:  RFC5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC also named activator 1 is a protein complex consisting of five distinct subunits of 140 40 38 37 and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9. [provided by RefSeq May 2011]

PrimePCR™ SYBR® Green Assay: RFC5, Human
replication factor C (activator 1) 5, 36.5kDa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043375
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050637
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PrimePCR™ SYBR® Green Assay: RFC5, Human
replication factor C (activator 1) 5, 36.5kDa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009107
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PrimePCR™ Probe Assay: RFC5, Human
replication factor C (activator 1) 5, 36.5kDa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050637
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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