Gene:  RBP3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity suggesting a series of gene duplication events. In the gene the boundaries of these domains are not defined by exon-intron junctions as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon which is 3180 base pairs long. The remainder of the fourth domain is encoded in the last three exons which are 191 143 and approximately 740 base pairs long respectively. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: RBP3, Human
retinol binding protein 3, interstitial

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015975
Info:   Same primer pair as used in probe assay qHsaCIP0030296
List Price:    $130.00
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PrimePCR™ Probe Assay: RBP3, Human
retinol binding protein 3, interstitial

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030296
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: RBP3, Human
retinol binding protein 3, interstitial

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021277
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015975; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RBP3, Human
retinol binding protein 3, interstitial

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053900
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030296; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: RBP3, Human
retinol binding protein 3, interstitial

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053901
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030296; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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