Gene:  RBFOX1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The Fox-1 family of RNA-binding proteins is evolutionarily conserved and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2011]

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PrimePCR™ SYBR® Green Assay: RBFOX1, Human
RNA binding protein, fox-1 homolog (C. elegans) 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037632
Info:   Same primer pair as used in probe assay qHsaCEP0040355
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PrimePCR™ SYBR® Green Assay: RBFOX1, Human
RNA binding protein, fox-1 homolog (C. elegans) 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057475
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0058580
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PrimePCR™ Probe Assay: RBFOX1, Human
RNA binding protein, fox-1 homolog (C. elegans) 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040355
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PrimePCR™ Probe Assay: RBFOX1, Human
RNA binding protein, fox-1 homolog (C. elegans) 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058580
Info:   Recommended - best efficiency
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PrimePCR™ ddPCR™ Copy Number Assay: RBFOX1, Human
RNA binding protein, fox-1 homolog (C. elegans) 1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500338
Info:   69nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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