Gene:  RASGRF1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx muscarinic receptors and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq Mar 2009]

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PrimePCR™ SYBR® Green Assay: RASGRF1, Human
Ras protein-specific guanine nucleotide-releasing factor 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042171
Info:   Same primer pair as used in probe assay qHsaCEP0049434
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PrimePCR™ SYBR® Green Assay: RASGRF1, Human
Ras protein-specific guanine nucleotide-releasing factor 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015743
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PrimePCR™ Probe Assay: RASGRF1, Human
Ras protein-specific guanine nucleotide-releasing factor 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049434
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PrimePCR™ ddPCR™ Mutation Assay:RASGRF1 p.P558P, Human
Ras protein-specific guanine nucleotide-releasing factor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502936
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:RASGRF1 WT for p.P558P, Human
Ras protein-specific guanine nucleotide-releasing factor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502937
Info:   62nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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