Gene:  PSMC4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base which contains 6 ATPase subunits and 2 non-ATPase subunits and a lid which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome the immunoproteasome is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with an orphan member of the nuclear hormone receptor superfamily highly expressed in liver and with gankyrin a liver oncoprotein. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: PSMC4, Human
proteasome (prosome, macropain) 26S subunit, ATPase, 4

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0041985
Info:   Same primer pair as used in probe assay qHsaCEP0049248
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PrimePCR™ SYBR® Green Assay: PSMC4, Human
proteasome (prosome, macropain) 26S subunit, ATPase, 4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011519
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PrimePCR™ Probe Assay: PSMC4, Human
proteasome (prosome, macropain) 26S subunit, ATPase, 4

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049248
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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