Gene:  PSAP, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A B C and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease Tay-Sachs disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PSAP, Human
prosaposin

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048228
Info:   Same primer pair as used in probe assay qHsaCEP0055488
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PrimePCR™ SYBR® Green Assay: PSAP, Human
prosaposin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0008979
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: PSAP, Human
prosaposin

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0055488
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PrimePCR™ ddPCR™ Copy Number Assay: PSAP, Human
prosaposin

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000178
Info:   62nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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