Gene:  PRKCI, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2 where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PRKCI, Human
protein kinase C, iota

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046101
Info:   Same primer pair as used in probe assay qHsaCEP0053362
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PrimePCR™ SYBR® Green Assay: PRKCI, Human
protein kinase C, iota

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0022507
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PrimePCR™ Probe Assay: PRKCI, Human
protein kinase C, iota

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0053362
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PrimePCR™ ddPCR™ Mutation Assay:PRKCI p.P118P, Human
protein kinase C, iota

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504912
Info:   94nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PRKCI WT for p.P118P, Human
protein kinase C, iota

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504913
Info:   94nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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