Gene:  PRDM16, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PRDM16, Human
PR domain containing 16

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045066
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052327
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: PRDM16, Human
PR domain containing 16

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010104
List Price:    $125.00
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PrimePCR™ Probe Assay: PRDM16, Human
PR domain containing 16

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052327
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PRDM16, Human
PR domain containing 16

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009644
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0045066; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PRDM16, Human
PR domain containing 16

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016842
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010104; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PRDM16, Human
PR domain containing 16

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5037598
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0052327; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PRDM16, Human
PR domain containing 16

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5037599
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052327; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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