Gene:  PPP2R1A, Human

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This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme which is composed of a catalytic subunit and a constant regulatory subunit that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq Apr 2010]

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PrimePCR™ SYBR® Green Assay: PPP2R1A, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010551
Info:   Same primer pair as used in probe assay qHsaCIP0027632
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PrimePCR™ Probe Assay: PPP2R1A, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027632
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PrimePCR™ ddPCR™ Copy Number Assay: PPP2R1A, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000049
Info:   74nt amplicon; intronic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.R258H, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500876
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.R258H, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500877
Info:   64nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.P179L, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502272
Info:   60nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.P179L, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502273
Info:   60nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.R182W, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503174
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.R182W, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503175
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.S256F, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503630
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.S256F, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503631
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.P179R, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504580
Info:   60nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.P179R, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504581
Info:   60nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.R183Q, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505916
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.R183Q, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505917
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.R183W, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505994
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.R183W, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505995
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A p.S256Y, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2506116
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PPP2R1A WT for p.S256Y, Human
protein phosphatase 2, regulatory subunit A, alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2506117
Info:   70nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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