Gene:  PPP1R12A, Human

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Myosin phosphatase target subunit 1 which is also called the myosin-binding subunit of myosin phosphatase is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase) which is activated by GTP. RhoA phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2009]

PrimePCR™ SYBR® Green Assay: PPP1R12A, Human
protein phosphatase 1, regulatory (inhibitor) subunit 12A

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011200
Info:   Same primer pair as used in probe assay qHsaCIP0027906
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PrimePCR™ Probe Assay: PPP1R12A, Human
protein phosphatase 1, regulatory (inhibitor) subunit 12A

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027906
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