Gene:  POMC, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a polypeptide hormone precursor that undergoes extensive tissue-specific post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and depending on tissue type and the available convertases processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin essential for normal steroidogenesis and the maintenance of normal adrenal weight and lipotropin beta are the major end products. In other tissues including the hypothalamus placenta and epithelium all cleavage sites may be used giving rise to peptides with roles in pain and energy homeostasis melanocyte stimulation and immune modulation. These include several distinct melanotropins lipotropins and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity adrenal insufficiency and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: POMC, Human
proopiomelanocortin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0038921
Info:   Same primer pair as used in probe assay qHsaCIP0041643
List Price:    $130.00
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PrimePCR™ Probe Assay: POMC, Human
proopiomelanocortin

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0041643
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: POMC, Human
proopiomelanocortin

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5025285
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0038921; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POMC, Human
proopiomelanocortin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5059256
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0041643; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POMC, Human
proopiomelanocortin

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5059257
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0041643; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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