Gene:  POLK, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases such as POLK permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al. 2007 [PubMed 17317631]).[supplied by OMIM Jan 2010]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: POLK, Human
polymerase (DNA directed) kappa

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043926
Info:   Same primer pair as used in probe assay qHsaCEP0051188
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: POLK, Human
polymerase (DNA directed) kappa

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010388
List Price:    $130.00
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Add to Custom Plate
PrimePCR™ Probe Assay: POLK, Human
polymerase (DNA directed) kappa

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051188
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: POLK, Human
polymerase (DNA directed) kappa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008634
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043926; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: POLK, Human
polymerase (DNA directed) kappa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5017067
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010388; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POLK, Human
polymerase (DNA directed) kappa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035580
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051188; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POLK, Human
polymerase (DNA directed) kappa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035581
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051188; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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