Gene:  POLH, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However it accurately replicates UV-damaged DNA; when thymine dimers are present this polymerase inserts the complementary nucleotides in the newly synthesized DNA thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV a variant type of xeroderma pigmentosum. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: POLH, Human
polymerase (DNA directed), eta

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016237
Info:   Same primer pair as used in probe assay qHsaCIP0030458
List Price:    $125.00
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PrimePCR™ Probe Assay: POLH, Human
polymerase (DNA directed), eta

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030458
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: POLH, Human
polymerase (DNA directed), eta

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021495
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0016237; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POLH, Human
polymerase (DNA directed), eta

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5054210
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030458; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: POLH, Human
polymerase (DNA directed), eta

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5054211
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030458; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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