Gene:  PMEL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes which are the melanin-producing organelles in melanocytes and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq Jan 2011]

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PrimePCR™ SYBR® Green Assay: PMEL, Human
premelanosome protein

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013129
Info:   Same primer pair as used in probe assay qHsaCIP0028738
List Price:    $125.00
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PrimePCR™ Probe Assay: PMEL, Human
premelanosome protein

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028738
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PMEL, Human
premelanosome protein

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019121
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013129; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PMEL, Human
premelanosome protein

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051204
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028738; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PMEL, Human
premelanosome protein

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051205
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028738; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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