Gene:  PKN1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PKN1, Human
protein kinase N1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016992
Info:   Same primer pair as used in probe assay qHsaCIP0030947
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PrimePCR™ Probe Assay: PKN1, Human
protein kinase N1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030947
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PKN1, Human
protein kinase N1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022147
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0016992; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: PKN1, Human
protein kinase N1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5055136
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030947; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: PKN1, Human
protein kinase N1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5055137
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030947; Intron-spanning
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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