Gene:  PIKFYVE, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions membrane trafficking and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(35)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq May 2010]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: PIKFYVE, Human
phosphoinositide kinase, FYVE finger containing

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017829
Info:   Same primer pair as used in probe assay qHsaCIP0031484
List Price:    $130.00
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PrimePCR™ Probe Assay: PIKFYVE, Human
phosphoinositide kinase, FYVE finger containing

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031484
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PIKFYVE, Human
phosphoinositide kinase, FYVE finger containing

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022808
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017829; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PIKFYVE, Human
phosphoinositide kinase, FYVE finger containing

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056126
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031484; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PIKFYVE, Human
phosphoinositide kinase, FYVE finger containing

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056127
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031484; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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