Gene:  PIK3CA, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit which uses ATP to phosphorylate PtdIns PtdIns4P and PtdIns(45)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: PIK3CA, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0023833
Info:   Same primer pair as used in probe assay qHsaCIP0033870
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PrimePCR™ Probe Assay: PIK3CA, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033870
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PrimePCR™ ddPCR™ Copy Number Assay: PIK3CA, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000048
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA p.E542K, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000073
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA WT for p.E542K, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000074
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA p.E545K, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000075
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA WT for p.E545K, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000076
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA p.H1047R, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000077
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA WT for p.H1047R, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000078
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA p.H1047L, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000123
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: PIK3CA WT for p.H1047L, Human
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000124
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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