Gene:  PICALM, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a clathrin assembly protein which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2011]

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PrimePCR™ SYBR® Green Assay: PICALM, Human
phosphatidylinositol binding clathrin assembly protein

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042344
Info:   Same primer pair as used in probe assay qHsaCEP0049607
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PrimePCR™ SYBR® Green Assay: PICALM, Human
phosphatidylinositol binding clathrin assembly protein

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010574
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: PICALM, Human
phosphatidylinositol binding clathrin assembly protein

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049607
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PrimePCR™ ddPCR™ Copy Number Assay: PICALM, Human
phosphatidylinositol binding clathrin assembly protein

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000220
 
Fluorophore:   FAM
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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