Gene:  PHKA1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Phosphorylase kinase is a polymer of 16 subunits four each of alpha beta gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq Feb 2010]

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PrimePCR™ SYBR® Green Assay: PHKA1, Human
phosphorylase kinase, alpha 1 (muscle)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042252
Info:   Same primer pair as used in probe assay qHsaCEP0049515
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: PHKA1, Human
phosphorylase kinase, alpha 1 (muscle)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015458
List Price:    $130.00
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PrimePCR™ Probe Assay: PHKA1, Human
phosphorylase kinase, alpha 1 (muscle)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049515
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PHKA1, Human
phosphorylase kinase, alpha 1 (muscle)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007199
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042252; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PHKA1, Human
phosphorylase kinase, alpha 1 (muscle)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020834
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015458; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PHKA1, Human
phosphorylase kinase, alpha 1 (muscle)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032710
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049515; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PHKA1, Human
phosphorylase kinase, alpha 1 (muscle)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032711
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049515; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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