Gene:  PFKL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis namely the conversion of D-fructose 6-phosphate to D-fructose 16-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits PFK from liver is a homotetramer of L-subunits while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq Aug 2008]

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PrimePCR™ SYBR® Green Assay: PFKL, Human
phosphofructokinase, liver

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013324
Info:   Same primer pair as used in probe assay qHsaCIP0028823
List Price:    $125.00
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PrimePCR™ Probe Assay: PFKL, Human
phosphofructokinase, liver

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028823
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PFKL, Human
phosphofructokinase, liver

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019252
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013324; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PFKL, Human
phosphofructokinase, liver

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051336
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028823; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PFKL, Human
phosphofructokinase, liver

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051337
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028823; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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