Gene:  PDK3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2010]

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PrimePCR™ SYBR® Green Assay: PDK3, Human
pyruvate dehydrogenase kinase, isozyme 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015707
Info:   Same primer pair as used in probe assay qHsaCIP0030112
List Price:    $130.00
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PrimePCR™ Probe Assay: PDK3, Human
pyruvate dehydrogenase kinase, isozyme 3

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030112
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PDK3, Human
pyruvate dehydrogenase kinase, isozyme 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021045
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015707; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PDK3, Human
pyruvate dehydrogenase kinase, isozyme 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053552
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030112; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: PDK3, Human
pyruvate dehydrogenase kinase, isozyme 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5053553
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030112; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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