Gene:  PDF, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

Why PrimePCR?

PrimePCR
Lookup Tool
Design and Validation of Real-Time PCR Primers
Pathway Curation
and Array Design Strategy
Control and Reference Assays for Real-Time PCR

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene peptide deformylase (PDF) removes the formyl group from the initiating methionine of nascent peptides. In eubacteria deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF actinonin acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq Nov 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: PDF, Human
peptide deformylase (mitochondrial)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048945
Info:   Same primer pair as used in probe assay qHsaCEP0056205
List Price:    $125.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ SYBR® Green Assay: PDF, Human
peptide deformylase (mitochondrial)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012452
Info:   Recommended - best specificity
List Price:    $125.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate
PrimePCR™ Probe Assay: PDF, Human
peptide deformylase (mitochondrial)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0056205
List Price:    $180.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PDF, Human
peptide deformylase (mitochondrial)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018645
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012452; Intron-spanning
List Price:    $125.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Expression EvaGreen® Assay: PDF, Human
peptide deformylase (mitochondrial)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189360
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0048945; exonic
List Price:    $125.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Expression Probe Assay: PDF, Human
peptide deformylase (mitochondrial)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192411
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0056205; exonic
 
Fluorophore:   FAM
List Price:    $190.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Expression Probe Assay: PDF, Human
peptide deformylase (mitochondrial)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192412
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0056205; exonic
 
Fluorophore:   HEX
List Price:    $190.00
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.