Gene:  PABPC1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.[provided by RefSeq Aug 2010]

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PrimePCR™ SYBR® Green Assay: PABPC1, Human
poly(A) binding protein, cytoplasmic 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056735
Info:   Same primer pair as used in probe assay qHsaCEP0057840
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PrimePCR™ SYBR® Green Assay: PABPC1, Human
poly(A) binding protein, cytoplasmic 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0019790
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: PABPC1, Human
poly(A) binding protein, cytoplasmic 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057840
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PrimePCR™ ddPCR™ Mutation Assay:PABPC1 p.I195V, Human
poly(A) binding protein, cytoplasmic 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503040
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:PABPC1 WT for p.I195V, Human
poly(A) binding protein, cytoplasmic 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503041
Info:   100nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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