Gene:  P4HB, Human

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This gene encodes the beta subunit of prolyl 4-hydroxylase a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner its ability to bind thyroid hormone its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: P4HB, Human
prolyl 4-hydroxylase, beta polypeptide

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002124
Info:   Recommended - best coverage
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PrimePCR™ SYBR® Green Assay: P4HB, Human
prolyl 4-hydroxylase, beta polypeptide

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0056728
Info:   Same primer pair as used in probe assay qHsaCEP0057833
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PrimePCR™ Probe Assay: P4HB, Human
prolyl 4-hydroxylase, beta polypeptide

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0057833
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: P4HB, Human
prolyl 4-hydroxylase, beta polypeptide

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5000900
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002124; Exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: P4HB, Human
prolyl 4-hydroxylase, beta polypeptide

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5013403
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0056728; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: P4HB, Human
prolyl 4-hydroxylase, beta polypeptide

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045116
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0057833; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: P4HB, Human
prolyl 4-hydroxylase, beta polypeptide

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5045117
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0057833; exonic
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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