Gene:  ORC1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle the levels of this protein vary during the cell cycle which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1) a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jun 2010]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: ORC1, Human
origin recognition complex, subunit 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0016725
Info:   Same primer pair as used in probe assay qHsaCIP0030768
List Price:    $130.00
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PrimePCR™ Probe Assay: ORC1, Human
origin recognition complex, subunit 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0030768
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ORC1, Human
origin recognition complex, subunit 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5021914
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0016725; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ORC1, Human
origin recognition complex, subunit 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5054796
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0030768; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ORC1, Human
origin recognition complex, subunit 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5054797
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0030768; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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