Gene:  OPN4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates but does not abolish photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: OPN4, Human
opsin 4

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037655
Info:   Same primer pair as used in probe assay qHsaCEP0040378
List Price:    $125.00
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PrimePCR™ Probe Assay: OPN4, Human
opsin 4

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040378
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: OPN4, Human
opsin 4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5006169
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037655; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: OPN4, Human
opsin 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5030650
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0040378; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: OPN4, Human
opsin 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5030651
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0040378; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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