Gene:  Obscn, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The obscurin gene spans more than 150 kb contains over 80 exons and encodes a protein of approximately 800 kDa. The encoded protein contains 68 Ig domains 2 fibronectin domains 1 calcium/calmodulin-binding domain 1 RhoGEF domain with an associated PH domain and 2 serine-threonine kinase domains. This protein is one of three giant sacromeric signaling proteins that includes titin and nebulin. It may have a role in the organization of myofibrils during assembly and also may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been described although the full-length nature is not known for all splicing variants. [provided by RefSeq Jan 2010]

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PrimePCR™ SYBR® Green Assay: Obscn, Mouse
Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0022299
Info:   Same primer pair as used in probe assay qMmuCIP0034340
List Price:    $125.00
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PrimePCR™ Probe Assay: Obscn, Mouse
Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCIP0034340
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Obscn, Mouse
Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5084096
Info:   EG; Same primer pair as used in qPCR assay qMmuCID0022299; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Obscn, Mouse
Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5119996
Info:   FAM; Same primer pair and probe as used in qPCR assay qMmuCIP0034340; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Obscn, Mouse
Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5119997
Info:   HEX; Same primer pair and probe as used in qPCR assay qMmuCIP0034340; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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