Gene:  NUB1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein that functions as a negative regulator of NEDD8 a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene which is associated with Leber congenital amaurosis an inherited retinopathy and mutations in that gene can abolish interaction with this protein which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies and in glial cytoplasmic inclusions in multiple system atrophy with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq Aug 2011]

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PrimePCR™ SYBR® Green Assay: NUB1, Human
negative regulator of ubiquitin-like proteins 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0041989
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049252
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: NUB1, Human
negative regulator of ubiquitin-like proteins 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009390
List Price:    $130.00
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PrimePCR™ Probe Assay: NUB1, Human
negative regulator of ubiquitin-like proteins 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049252
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NUB1, Human
negative regulator of ubiquitin-like proteins 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5006960
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0041989; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NUB1, Human
negative regulator of ubiquitin-like proteins 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016356
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009390; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NUB1, Human
negative regulator of ubiquitin-like proteins 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032232
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0049252; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NUB1, Human
negative regulator of ubiquitin-like proteins 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5032233
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0049252; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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