Gene:  NOB1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
Pathway Curation
and Array Design Strategy
Control and Reference Assays for Real-Time PCR

In yeast over 200 protein and RNA cofactors are required for ribosome assembly and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S 5.8S and 25S rRNAs folding of the rRNA and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein 2009 [PubMed 19706509]).[supplied by OMIM Nov 2010]

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PrimePCR™ SYBR® Green Assay: NOB1, Human
NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009439
Info:   Same primer pair as used in probe assay qHsaCIP0027193
List Price:    $125.00
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PrimePCR™ Probe Assay: NOB1, Human
NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027193
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NOB1, Human
NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016394
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009439; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NOB1, Human
NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048584
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027193; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NOB1, Human
NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048585
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027193; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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