Gene:  NMT1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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Myristate a rare 14-carbon saturated fatty acid is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al. 1992 [PubMed 1570339]).[supplied by OMIM Nov 2008]

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PrimePCR™ SYBR® Green Assay: NMT1, Human
N-myristoyltransferase 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006258
Info:   Same primer pair as used in probe assay qHsaCIP0026086
List Price:    $125.00
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PrimePCR™ Probe Assay: NMT1, Human
N-myristoyltransferase 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026086
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NMT1, Human
N-myristoyltransferase 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014325
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0006258; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NMT1, Human
N-myristoyltransferase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046630
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0026086; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NMT1, Human
N-myristoyltransferase 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046631
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026086; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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