Gene:  NFX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants each of which encodes a different isoform have been identified. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: NFX1, Human
nuclear transcription factor, X-box binding 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006506
Info:   Same primer pair as used in probe assay qHsaCIP0026193
List Price:    $134.00
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PrimePCR™ Probe Assay: NFX1, Human
nuclear transcription factor, X-box binding 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0026193
List Price:    $196.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NFX1, Human
nuclear transcription factor, X-box binding 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014512
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0006506; Intron-spanning
List Price:    $134.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NFX1, Human
nuclear transcription factor, X-box binding 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046818
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0026193; Intron-spanning
 
Fluorophore:   FAM
List Price:    $201.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NFX1, Human
nuclear transcription factor, X-box binding 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5046819
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0026193; Intron-spanning
 
Fluorophore:   HEX
List Price:    $201.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download