Gene:  NFS1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq Nov 2010]

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PrimePCR™ SYBR® Green Assay: NFS1, Human
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0048798
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0056058
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: NFS1, Human
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013433
List Price:    $125.00
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PrimePCR™ Probe Assay: NFS1, Human
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0056058
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NFS1, Human
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5012980
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0048798; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: NFS1, Human
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019329
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013433; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NFS1, Human
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5044270
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0056058; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: NFS1, Human
NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5044271
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0056058; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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